TABLE 1.
Primers used for generating TB40/E-mCherry-US28wt (US28wt)b
| Recombinant virus (TB40/E-mCherry) | Primer descriptiona | Primer sequence (5′–3′) |
|---|---|---|
| US27Δ-galK insertion | FOR | GTGTAATGCTTTTTACAGGACCGTTCAACAGGTGATACTACCTGCAAGGTACCTGTTGACAATTAATCATCGGCA |
| REV | CGTGCAATTAGCAAAAATAGATGTGCGGCGGACGCGTGAGAGAGGATCGAATCAGCACTGTCCTGCTCCTT | |
| US27Δ | DS oligonucleotide | TTTACAGGACCGTTCAACAGGTGATACTACCTGCAAGGTATTCGATCCTCTCTCACGCGTCCGCCGCACATCTATTTTTG |
| UL33Δ-galK insertion | FOR | TTCCGCCCAGACCCGCAACAACACTCCTCCGCACATCAATGACACTTGCAACCCTGTTGACAATTAATCATCGGCA |
| REV | GGGGAAATGGCGACGGGTTCTGGTGCTTTCTGAATAAAGTAACAGGAAAGCTCAGCACTGTCCTGCTCCTT | |
| UL33Δ | DS oligonucleotide | CCGCAACAACACTCCTCCGCACATCAATGACACTTGCAACGCTTTCCTGTTACTTTATTCAGAAAGCACCAGAACCCGTC |
| UL78Δ-galK insertion | FOR | GTCCCCGGAGAGGGTATATTCGTTCGGCGAGAGCGGGCGGCGGTGGTGGGTCCTGTTGACAATTAATCATCGGCA |
| REV | TAACGTGATTTATCTGCCACTTTTCTCCCCGCTGCCGTACAGCGCCGCCGCTCAGCACTGTCCTGCTCCTT | |
| UL78Δ | DS oligonucleotide | GGGTATATTCGTTCGGCGAGAGCGGGCGGCGGTGGTGGGTGCGGCGGCGCTGTACGGCAGCGGGGAGAAAAGTGGCAGAT |
a
FOR, forward; REV, reverse; DS, double-stranded.
b
Boldface sequences are complementary to the galK template plasmid.