FIG 2.

MC159 induces NF-κB-dependent reporter gene expression in transfected cells. (A) NIH 3T3 cells and 293A cells were cotransfected with expression plasmids as indicated and the reporter plasmid pNiFty-SEAP. Thirty hours posttransfection, Quanti-Blue reagent was added to the supernatant and SEAP activity was quantified colorimetrically. Results were normalized to empty vector (pcDNA). Cells were harvested and proteins analyzed by immunoblotting. (B) NIH 3T3 cells were cotransfected with decreasing amounts of MC159 and constant amounts of the reporter plasmid pNiFty-SEAP. Results were normalized to pEGFP. The experiment was carried out as described for panel A. Means ± SEM for experiments done in triplicate are shown. ***, P < 0.001.