FIG 3.

MCMV ΔM45::MC159 promotes degradation of IκBα and translocation of p65 into the nucleus. (A to C) NIH 3T3 cells were infected with MCMV M45HA, M45Ct3, or ΔM45::MC159 at an MOI of 5 TCID₅₀/cell. Cells were lysed at the indicated times postinfection, and protein levels were analyzed by immunoblotting. (D to F) NIH 3T3 cells were infected with an MOI of 2 TCID₅₀/cell. Cells were fixed at the indicated times postinfection, and the subcellular localization of the NF-κB subunit p65 was analyzed by immunofluorescence. Costaining with HA served as an infection control. Nuclei were stained with DRAQ5. Arrowheads indicate cells with nuclear p65.