FIG 9.

Glycan microarray analysis of mutant HA proteins. Glycan microarrays containing nonsialylated controls (glycans 1 and 2), α2,3-linked sialosides (red bars, glycans 3 to 35), α2,6-linked sialosides (blue bars, glycans 36 to 56), and glycans possessing both α2,3- and α2,6-linked sialic acids (checkered bars, glycans 57 and 58); a complete list of glycans is provided in Table S1 in the supplemental material. Viruses were evaluated for their ability to bind to the respective sialosides (relative fluorescence units). A “+” indicates that the HA protein encodes the respective amino acid change(s) in the background of H5HA-N224K+Q226L.