FIG 4.

Increasing salt concentrations (KCl) diminished MARV VP40 association with anionic membranes. (A) SDS-PAGE of supernatant (SN) and pellet (P) fractions collected from MLV sedimentation assays that were performed in buffers that contained different KCl concentrations, as noted above the gel. All the liposomes consisted of POPC-POPE-POPS (40:30:30). (B) Quantification of MLV sedimentation assay results. The bars represent fractions of MARV VP40 associated with MLVs under different concentrations of KCl. The data represent the averages of three sedimentation assays, with the error bars representing the standard errors of the mean. *, P < 0.05.