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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Trends Immunol. 2015 Apr 27;36(5):315–322. doi: 10.1016/j.it.2015.03.007

Figure 2. A pooled approach to in vivo T cell screens using arrayed transduction of shRNAs.

Figure 2

(A) Screens based on pooling cells after transduction with unique shRNAs in arrays. DNA for viral shRNA constructs is used to package virus and transduce query cells at high-efficiency (high MOIs) in arrayed format and cells are pooled before transfer in vivo [10].

(B) FACS-based recovery of all transferred cells after subsetting based on differentiation-associated phenotypes.

(C) Next generation sequencing libraries amplified from proviral DNA encoding shRNA sequences are prepared from genomic DNA samples of input and sorted cell populations and differential shRNA representation is quantified after high-throughput sequencing.