Sub-fractioning anti-inflammatory components of American ginseng crude extract. (A) Cytotoxicity of American ginseng crude extracts (Am. G), hexane fraction (Hexane-F), water-F, butanol-F, dichloremethane-F, ethyl acetate-F, and maltodextrin control in RAW264.7 cells. Sub-confluent RAW264.7 cells were treated with American ginseng crude extracts and other fractions in DMEM supplemented with 2% FBS as indicated for 24 h. n=6, *p<0.05 vs. control (0) in each group. (B) The effects of American ginseng crude extracts (Am. G), hexane fraction (Hexane-F), water-F, butanol-F, dichloremethane-F, ethyl acetate-F, and maltodextrin control on iNOS expression in LPS-inflamed RAW264.7 cells. Sub-confluent RAW264.7 cells were treated with LPS, American ginseng crude extracts and other fractions in DMEM supplemented with 2% FBS for 6 h. The immunoblotting results are representatives of three separate experiments.