Fig. 5.
American ginseng crude extract- and sub-fractions-operated Nrf2 transcriptional activity in RAW264.7 cells. (A) The effects of American ginseng crude extracts, hexane fraction, and panaxynol on Nrf2 Neh2-luciferase (Luc) activity in RAW264.7 cells. Sub-confluent RAW264.7 cells transfected with Nrf2 Neh2-Luc and TK-Luc were treated with American ginseng crude extracts (Am. G, 100 μg/ml), hexane fraction (Hexane-F, 100 μg/ml), and panaxynol (1 μM) in DMEM supplemented with 2% FBS for 6 h. n=6, *p<0.05 vs. control (−). (B) The effects of American ginseng crude extracts, hexane fraction, and panaxynol on Nrf2 ARE-luciferase (Luc) activity in RAW264.7 cells. Sub-confluent RAW264.7 cells transfected with Nrf2 ARE-Luc and TK-Luc were treated with American ginseng crude extracts (Am. G, 100 μg/ml), hexane fraction (Hexane-F, 100 μg/ml), and panaxynol (1 μM) in DMEM supplemented with 2% FBS for 6 h. n=6, *p<0.05 vs. control (−).