TABLE 1.

Kinetics of pulmonary immune cells after intranasal IL-7–mFc treatment

Cells	Absolute cell numbers on day after IL-7–mFc treatmenta:
	0	3	7	14	21	35
Total CD4 T (×106)	0.68 ± 0.07	0.76 ± 0.03	2.08 ± 0.28**	0.89 ± 0.19	0.79 ± 0.14	0.70 ± 0.07
CD62Llo CD44high CD4 T (×105)	0.58 ± 0.08	1.44 ± 0.08**	7.80 ± 1.24**	1.61 ± 0.25**	0.93 ± 0.05**	0.69 ± 0.05
Total CD8 T (×106)	0.31 ± 0.07	0.32 ± 0.01	0.75 ± 0.11**	0.42 ± 0.08	0.36 ± 0.08	0.28 ± 0.03
CD62Llo CD44high CD8 T (×105)	0.18 ± 0.03	0.29 ± 0.02*	1.22 ± 0.14**	0.50 ± 0.12*	0.25 ± 0.03	0.26 ± 0.04
γδ T (×105)	0.24 ± 0.02	0.39 ± 0.01**	4.71 ± 0.59**	0.54 ± 0.16**	0.40 ± 0.06*	0.38 ± 0.02**
B (×106)	0.84 ± 0.17	0.97 ± 0.03	2.27 ± 0.38*	1.13 ± 0.10	0.83 ± 0.18	ND
NK (×106)	0.36 ± 0.03	0.58 ± 0.03*	0.71 ± 0.07**	0.39 ± 0.04	0.29 ± 0.05	ND

^aMice received 1 μg of IL-7–mFc intranasally at 0, 3, 7, 14, and 21 days prior to sacrifice. Absolute numbers of immune cells in the total lung homogenates at each indicated time point were calculated based on the percentage of total cell number with flow cytometry. The results are representative of two independent experiments and are expressed as the mean ± SEM for four mice per group. ND, not determined. *, P < 0.05; **, P < 0.01 (by Student's t test, compared with cell numbers on day 0).