FIG 4.

ISVP-induced hemolysis of untreated and protease-treated RBCs. T3D (A) or T1L (B) ISVPs (2 × 10¹² particles/ml) were incubated in a 3% (vol/vol) solution of untreated or protease-treated bovine RBCs in the absence or presence of 300 mM CsCl for 2 h at 37°C. After 2 h, hemolysis was quantified by measuring the absorbance of the supernatant at 405 nm. Levels of 0 and 100% hemolysis were determined by incubating an equivalent number of RBCs in virus storage buffer or virus storage buffer supplemented with 0.8% Triton X-100, respectively, for 2 h at 37°C. For each condition, the mean percent hemolysis was determined from at least three independent experiments. Error bars indicate standard deviations. P values were calculated using Student's t test. *, P ≤ 0.05.