FIG 5.

Liposomes that mimic the composition of the early or late endosomal membranes facilitate ISVP-to-ISVP* conversion. (A) Lipid compositions of the EE and LE liposomes. (B) EE or LE liposome-mediated ISVP-to-ISVP* conversion. T3D ISVPs (2 × 10¹² particles/ml) were incubated in virus storage buffer supplemented with 1 mM EE or LE liposomes for 1 h at the indicated temperatures. After 1 h, the reaction mixtures were treated with trypsin (0.08 mg/ml) for 30 min on ice. Following digestion, the reaction mixtures were solubilized in reducing SDS sample buffer and analyzed by SDS-PAGE. The gels were Coomassie stained. The migration of the λ1,2,3 and δ bands is indicated to the left of each panel. The migration of molecular mass markers is indicated to the right of each panel.