FIG 7.

Liposomes composed of PC and PC-PA (5:1) promote ISVP-to-ISVP* conversion. T3D ISVPs (2 × 10¹² particles/ml) were incubated in virus storage buffer supplemented with 1 mM PC, PC-PA (5:1), or PC-sulfatide (7:1) liposomes for 1 h at the indicated temperatures. After 1 h, the reaction mixtures were treated with trypsin (0.08 mg/ml) for 30 min on ice. Following digestion, the reaction mixtures were solubilized in reducing SDS sample buffer and analyzed by SDS-PAGE. The gels were Coomassie stained. The migration of the λ1,2,3 and δ bands is indicated to the left of each panel. The migration of molecular mass markers is indicated to the right of each panel.