Host cell protein shutoff. Vero E6 cells were infected with rOROV, rOROVdelNSm, rOROV2080S, rOROVdelNSs, or rOROV246NSs or mock infected. Cells were infected at an MOI of 3 and incubated at 37°C. At the indicated time points, the cells were labeled with [35S]methionine for 2 h. Cells lysates were then separated by SDS-PAGE. Arrows indicate the position of viral proteins Gc, N, NSm, and NSs.