FIG 5.

Biological interferon production assay. A549 cells were infected at an MOI of 1 with BUNV, rBUNdelNSs2, rOROV, rOROVdelNSm, rOROV2080S, rOROVdelNSs, or rOROV246NSs or mock infected. Supernatant was harvested at 24 h p.i., and cell extracts were separated by SDS-PAGE. (A) UV-inactivated supernatant was used to pretreat A549-N pro cells prior to infection with EMCV. At 3 days p.i., cells were fixed and stained with crystal violet. (B) Graph calculated from panel A, presenting relative IFN units expressed as 2N where N is the number of 2-fold dilutions that offered protection. (C) Cells extracts were probed for OROV N, STAT1, pSTAT1, and MxA. Tubulin was probed as a loading control.