FIG 10.

mLANA is necessary for p53 inactivation during MHV68 replication. 3T3 fibroblasts were mock infected or infected with WT MHV68 or mLANA-null MHV68 (73.STOP) at an MOI of 5 PFU/cell. Cells were treated with doxorubicin (1 μM) or vehicle (DMSO) 14 h postinfection, and lysates were harvested in RIPA buffer 18 h postinfection. Proteins were resolved by SDS-PAGE. Resolved proteins were detected by immunoblot analyses using antibodies to detect the indicated proteins. Detection of β-actin serves as a loading control.