TABLE 1.

Primers used in this study

Primer	Sequence (5′→3′)a	Restriction site	Locationb
gDfw	CAC AGA ATT CAC CTG CCA GCG CCA TGC	EcoRI	121132–121148
gDrv	CAC AGA ATT CCA TCG ACG CCG GTA CTG C	EcoRI	122370–121353
gBmutΔK883/008R	CCG AAT TCC TAC CCG CTG TTC TTG CGC GC	EcoRI	16138–16157
gBmutE290G F	CGT CGA GGA GGT GGG GGC GCG CTC CG		17943–17918
gBmutE290G R	CG GAG CGC GCC CCC ACC TCC TCG ACG		17918–17943
gBmutG672R F	CGC TAC TTT AAG CTG AGG AGC GGG TAC G		16799–16772
gBmutG672R R	C GTA CCC GCT CCT CAG CTT AAA GTA GCG		16772–16799
gDmut V25A-fwd	G GAC GCC GTG CCC GCG CCG ACC TTC CCC C		121164–121173
gDmut V25A-rev	G GGG GAA GGT CGG CGC GGG CAC GGC GTC C		121173–121164
gDmut A25V-fwd	G GAC GCC GTG CCC GTG CCG ACC TTC CCC C		121164–121173
gDmut A25V-rev	G GGG GAA GGT CGG CAC GGG CAC GGC GTC C		121173–121164
gDmut V106A-fwd	GCG GAC GGG TGC GCG CAC CTG CTG TAC		121247–121255
gDmut V106A-rev	GTA CAG CAG GTG CGC GCA CCC GTC CGC		121255–121247
gDmut A106V-fwd	GCG GAC GGG TGC GTG CAC CTG CTG TAC		121247–121255
gDmut A106V-rev	GTA CAG CAG GTG CAC GCA CCC GTC CGC		121255–121247

^aNucleotide substitutions are underlined, and added restriction sites are in bold.

^bPrimer sequence locations in the PrV-Ka genome corresponding to accession number JF797218.1 (34) for gB primers or JQ809328.1 (35) for gD and gH primers.