FIG 11.

MHV-A59 infection during the chronic phase did not alter Cx43 protein expression in vivo. Mice were infected intracranially with MHV-A59 at 50% of the LD₅₀ or mock infected with PBS-BSA. Mock-infected and virus-infected mice were sacrificed at day 30 p.i., and total protein was extracted from brain tissue. Twenty micrograms of protein was resolved in SDS-PAGE, followed by transfer to a PVDF membrane and probing for Cx43 or the internal control, γ-actin. No observable alteration was observed. Data from two representative infected mice are shown.