FIG 2.

Expression of Cx43 at the cell surface of GFAP⁺ primary astrocytes. Primary astrocytes were double immune labeled with mouse anti-GFAP (astrocyte marker) and rabbit anti-Cx43 antisera. Cells subsequently were labeled with FITC goat anti-mouse IgG and Texas Red goat anti-rabbit IgG, respectively. Immunostained cells were counterstained with DAPI (blue). Merged images show that GFAP-positive astrocytes express high levels of punctate Cx43 staining at the cell surface. Insets show magnifications of the expression of Cx43 at the cell surface of GFAP⁺ astrocytes.