FIG 5.

Localization of Cx43 predominantly in the ER/ERGIC of virus-infected cells. Primary astrocytes were mock infected or infected with MHV-A59 at an MOI of 2 and were subjected to double-label immunofluorescence at 24 h p.i. with anti-Cx43 antisera (red) and anti-calnexin (green) or anti-β-cop antisera(green). The images show prominent punctate staining of Cx43 at the cell surface (A and C, thin arrow), forming gap junction plaques, in mock-infected cells. Cx43 in the virus-infected cells, which was retained in the intracellular compartments, mostly colocalized with the ER marker calnexin and/or ERGIC marker β-cop (B and D, thick arrow).