FIG 3.
Immature and mature T=3 HAstV-1 models fit into HAstV cryo-EM maps. (A) Immature T=3 HAstV-1 model fit into HAstV cryo-EM map (left) and a zoomed-in, sliced view (right). (B) Mature T=3 HAstV-1 model fit into HAstV cryo-EM map (left) and a zoomed-in, sliced view (right). Models are shown as cartoon views and are colored as in Fig. 1, and cryo-EM maps are shown as gray transparent surfaces. An oval, a triangle, and a pentagon indicate the 2-fold, 3-fold, and 5-fold icosahedral axes, respectively. For immature HAstV, contour levels were set to encompass the expected volume of 180 copies of VP70 plus 1 copy of the viral RNA genome, i.e., ∼18.5 × 106 Å3. For mature HAstV, contour levels were set to encompass the expected volume of 180 copies of VP34, 60 copies of VP25, and 1 copy of the viral RNA genome, i.e., ∼14.8 × 106 Å3. Calculations of volume values are based upon a protein density of 1.35 g/cm3 and an RNA density of 2.0 g/cm3.