FIG 4.

Structural and electrostatic comparisons between CP core domains from HAstV-1 and HEV. (A) Superposition of the HAstV-1 and HEV CP core domains. The HAstV-1 CP core is colored as in Fig. 1B, and the HEV CP core is colored gray. Significant differences between structures are indicated with arrows. (B and C) Electrostatic potential on the inner surface of the T=3 HAstV-1 CP core model (B) and the T=3 HEV CP core model (C). In the HAstV-1 model, the Cys82-Cys254 disulfide bond is colored yellow to show its proximity to electropositive residues. Note that in the T=3 HAstV-1 CP core model, the first 79 amino acids of the CP are not included. Similarly, in the T=3 HEV CP core model, the first 129 amino acids of the CP are not included. Thus, the electropositive surface comes only from amino acids on the inner core domain (S domain). Figures were made with Chimera (40).