FIG 1.

CCTA scanning mutagenesis of HCMV gH/gL. Schematic representation of HCMV gH (A) and gL (B) indicating the starting residue of each mutated charged cluster. The designation of gH domains D-I to D-IV was based on homology with the domain designations of EBV gH described by Matsuura et al. (26). Also indicated are the predicted signal peptides (sp), the transmembrane (tm) region of gH, the linear epitope of the neutralizing anti-gH MAb AP86 (33), and cysteine 144 of gL, which was reported by Ciferri et al. to form a disulfide linkage with either gO or UL128 (27). N-term, N terminus; aa, amino acids.