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Heterodimerization of gH and gL mutants. Human glioblastoma (U373) cells were transduced with the indicated combination of Ad vectors expressing wild-type (A) or mutant (B to D) gH and gL. Cell extracts were immunoprecipitated (IP) with MAb 14-4b or AP86, separated by reducing SDS-PAGE, and identified by immunoblotting with anti-gH or anti-gL antibodies. Mass standards (in kilodaltons) are indicated to the left of each panel.
