FIG 5.

Effect of gH/gL mutation on assembly of gH/gL/UL128-131. (A) ARPE-19 epithelial cells expressing wild-type or mutant gH/gL heterodimers alone or together with UL128-131 proteins were inoculated with HCMV, and infection was assessed by immunofluorescence detection of immediate early gene expression. (B) Relative HCMV infection was calculated as the percentage of HCMV-infected cells when cells expressed wild-type or mutant gH/gL plus UL128-131 divided by the percentage of HCMV-infected cells when cells expressed only the wild-type or mutant gH/gL dimer. Black bars, gH/gL mutants that were positive for promoting gB-mediated cell-cell fusion; white bars, mutants that were negative for fusion. Representative mutant gH/gL/UL128-131 complexes were tested in triplicate, and error bars represent standard errors.