FIG 6.

Coimmunoprecipitation and surface expression of H.671/gL/UL128-131. (A) ARPE-19 cells expressing either wild-type gH/gL/UL128-131 or H.671/gL/UL128-131 (or H.671/gL/UL128-131 lacking UL130) were immunoprecipitated with the anti-gH antibody AP86. Samples were separated by SDS-PAGE and analyzed by immunoblotting. Mass markers (in kilodaltons) and protein labels are displayed on the left and right, respectively. H.wt and L.wt, wild-type gH and gL, respectively. (B) Surface expression of wild-type gH/gL and H.671/L when expressed with UL128-131, measured by CELISA. Bars display the amount of surface gH as a percentage of the total amount of gH. Horizontal dashed line, the threshold for surface expression, as defined by the surface detection of gH expressed without gL, Error bars represent standard errors from three experiments.