FIG 1.

The NAE inhibitor MLN4924 inhibits Vpu-mediated downmodulation of CD4, but not Nef-mediated downmodulation of CD4 or Vpu-mediated downmodulation of BST2 in HeLa cells. HeLa cells were transfected to express Vpu or Nef, along with GFP as a transfection marker. The next day, cells were incubated with either DMSO alone or 500 nM MLN4924 in DMSO for 4 h, stained for surface CD4 or BST2, and analyzed by flow cytometry, gating on the GFP-positive populations. The results shown are representative of three experiments.