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A dominant negative mutant of cullin1 inhibits Vpu-mediated downmodulation of CD4 but not Vpu-mediated downmodulation of BST2 in HeLa cells. HeLa cells were transfected to express Vpu or Nef, either with or without a dominant negative version of cullin1, along with GFP as a transfection marker. The next day, cells were analyzed for surface CD4 or BST2 by flow cytometry, gating on the GFP-positive populations. The results shown are representative of three experiments.
