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. 2016 Feb 11;90(5):2486–2502. doi: 10.1128/JVI.02736-15

FIG 3.

FIG 3

MLN4924 inhibits Vpu-mediated downmodulation of CD4 and increases the surface expression of CD4-induced epitopes in Env. (A) 293-CD4-tetHIV-WT and -Δnef cells were treated with doxycycline to induce the expression of HIV-1 and incubated with either DMSO alone or 500 nM MLN4924 in DMSO for 4 h. The surface CD4 and internal HIV-1 p24 levels were determined by flow cytometry. Histograms represent “live” populations based on forward- and side-scatter; over 90% of live cells expressed p24 (not shown). (B) 293-CD4-tetHIV-Δnef cells were treated with doxycycline to induce the expression of HIV-1, and surface CD4 and specific Env epitopes were analyzed by flow cytometry, gating on the “live” populations. The color code applies to both panels A and B. The results shown are representative of two experiments.