FIG 7.

MLN4924 upregulates BST2 in interferon-treated cells expressing wild-type virus but minimally upregulates surface Env as measured by 2G12. (A) CEM.NKR.luc cells were infected with wild-type or vpu-negative (ΔVpu) HIV-1 as indicated. Three days later, the cells were treated or not with 30 ng/ml IFN-α, MLN4924, or both and then stained and analyzed the next day for surface BST2 and Env (using antibody 2G12), gating on p24-positive cells. The black dashed lines in the “wild-type” panels are the curves for ΔVpu in the absence of both interferon and MLN4924 for comparison. (B) For three independent experiments, the results for one of which are shown in panel A, the mean fluorescence intensities for BST2 and 2G12 were averaged and normalized to Δvpu in the absence of interferon or MLN4924, which was set to 100%. −, open bars indicate the absence of MLN4924; +, filled bars indicate treatment with MLN4924. Error bars indicate ±standard deviations.