FIG 8.

W76 in Vpu contributes to the modulation Env in infected T cells. CEM.NKR.luc cells were infected with wild-type, vpu-negative, or the indicated vpu mutant viruses as indicated. Three days later, the cells were treated or not with 30 ng/ml IFN-α, MLN4924, or both and then stained and analyzed the next day for surface Env (using antibody 2G12), gating on p24-positive cells. (B) For two independent experiments, the results for one of which are shown in panel A, the mean fluorescence intensities for 2G12 were averaged and normalized to Δvpu in the absence of interferon or MLN4924, which was set to 100%. −, open bars indicate the absence of MLN4924; +, filled bars indicate treatment with MLN4924. Error bars indicate ±standard deviations.