FIG 1.

Confocal micrographs of dissociated neurons infected with either US9 deletion (US9^—) or US9 repair (US9R) virus showing the distribution of glycoproteins gC, gD, and gE in the cell body periphery and along axons (arrows). Neonatal rat DRG neurons were dissociated, pelleted through a 35% Percoll gradient, and plated on Matrigel-coated coverslips. HSV-1-infected cultures were fixed at 24 hpi, permeabilized, and labeled for gC, gD, or gE. Deletion of pUS9 does not inhibit axonal transport of glycoproteins gC, gD, and gE. Bars, 10 μm.