FIG 5.

Virus spread from axons to nonneuronal Vero cells in the axonal compartment of neuronal cultures grown in microfluidic devices, after infection with US9 deletion (US9⁻ and F-US9/GFP) and US9 repair (US9R and F-US9/GFP-R) viruses. Foscarnet (100 μg/ml) was added to the axonal compartment 8 hpi, and cultures were fixed at 22 hpi followed by immunostaining for ICP27. The total number of ICP27-positive Vero cells in the axonal compartment was counted for each virus in three independent experiments.