TABLE 1.

Summary of positive immunofluorescence staining results for glycoproteins gC, gD, and gE along axons of rat dissociated neurons infected with F strain, KOS, or US9 deletion and repair viruses at 24 hpi

Virus	Presence of glycoproteins gC, gD, and gE alonga:
	Proximal axons	Mid- to distal axons
F strain	+++	++
KOS	+++	++
US9−	+++	++
US9R	+++	++
F-US9/GFP	+++	++
F-US9/GFP-R	+++	++

^aThe intensity of fluorescence signal along axons was compared to the intensity of fluorescence observed in the cell body. The intensity was classified semiquantitatively using the following criteria: +++, ≥intensity of the membrane of the cell body (this region shows the brightest fluorescence); ++, intensity the same as the cytoplasm of the cell body. Approximately 90% of infected neurons had one or more positive axons for glycoproteins. An average of 200 neurons were manually counted per virus in replicate experiments.