FIG 1.

Liposome binding analysis of purified Gag proteins. LUVs were prepared with the following compositions: POPC/POPS/Chol (34/30/36) and POPC/POPS/Chol/PI(4,5)P₂ (32/30/36/2). (A) Schematic of all purified proteins used (left) and representative Coomassie-stained polyacrylamide gel of flotation results (right). The asterisks indicate the relative locations of the I475A mutations. MB, membrane-bound fractions; NMB, non-membrane-bound fractions. (B) The bars represent the average percentages of total protein associated with liposomes for no less than four reactions; the error bars represent standard deviations.