FIG 5.

Schematic representation of the virus entry proximity ligation assay (VEPLA). Antibodies to UL37 and dynein are reacted with secondary antibodies linked to proximity probe primers, that after annealing to connector primers and amplification by a rolling-circle mechanism are detected by a DNA-intercalating fluorescent dye. A similar strategy was used to detect gD binding to nectin-1, representing virions attached to cell surfaces (not shown).