FIG 6.

Kinetics of virus entry into Vero cells. Confluent monolayers of Vero cells seeded in microscopy chamber slides were infected at an MOI of 10 with wild-type virus HSV-1(F) and tested by VEPLA for cell surface-bound virions (anti-gD/anti-nectin-1 antibodies) and cytoplasmic capsids at different times postinfection at 37°C (anti-UL37/antidynein). Anti-gM and antidynein antibodies were utilized as negative controls. The microtubules were visualized using mouse monoclonal antibody against α-tubulin (FITC), shown in green. DAPI (blue) was used for visualization of the nucleus, and the PLA signals were seen as red spots.