FIG 7.

McKrae gKΔ31–68 virions enter more slowly than parental McKrae virions in Vero cells. VEPLA was utilized to detect wild-type McKrae and gKΔ31–68 entry into Vero cells. Confluent Vero cell monolayers were infected with either McKrae or gKΔ31–68 viruses at an MOI of 10, and capsids that entered the cytoplasm and virions bound to cell surfaces were detected at 1 h postinfection with anti-UL37/antidynein and anti-gD/anti-nectin-1 antibodies, respectively. The PLA signals were quantified using SlideBook 5 digital imaging software. The efficiency of virus entry was calculated as E = number of fluorescent spots × intensity (UL37/dynein PLA)/number of spots × intensity (gD/nectin-1 PLA); E (gKΔ31–68)/E (McKrae) = 0.58.