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. 2016 Feb 11;90(5):2230–2239. doi: 10.1128/JVI.02468-15

FIG 8.

FIG 8

The McKrae gKΔ31–68 mutant virus is unable to enter DRG axons. VEPLA was utilized to asses McKrae and gKΔ31–68 virus entry into DRG neurons in cell culture. Embryonic day 18 (E8) rat DRGs were collected and seeded on polylysine-coated microscopy slides. DRGs were infected with WT HSV-1 strain McKrae or gKΔ31–68 at 6 days postseeding. The top and bottom left panels show interaction between gD and nectin-1 on DRG axons infected with WT McKrae and gKΔ31–68 mutant viruses, respectively, as seen by the presence of red spots along the axons (arrows). The top right panel shows the interaction between UL37 and dynein in DRG axons infected with WT McKrae virus. There was no interaction detected between UL37 and dynein in DRG axons infected with gKΔ31–68 (bottom right panel). Neurofilament marker (green) and DAPI (blue) were used to identify axons and the nuclei of glial cells, respectively.

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