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Genome organization of chimeric CHIKV/SFV viruses. Genome organization of chimeric CHIKV/SFV viruses. The different domains of E2 from SFV were inserted into the CHIKV genome in the corresponding position in individual constructs using a PCR-based cloning approach. Each virus expressed the GFP protein under the control of a second subgenomic promoter. Red portions of the E2 represent genetic sequences of SFV, whereas CHIKV is shown in blue.
