FIG 1.

SINV mutator RdRp presents altered replication kinetics at high-MOI passage in cell culture. (A and B) Growth curve of SINV-WT (solid line) and SINV-G (dashed line) at MOIs of 1 (A) and 25 (B). Mean values and standard errors of the means are shown (n = 3). Data were analyzed by two-way analysis of variance with Bonferroni's posttest (no significant differences were determined). (C and D) SINV-WT (solid line) and SINV-G (dashed line) were passaged 10 times in BHK-21 cells at MOIs of 1 (C) or 25 (D), and virus titers were measured by plaque assay. Mean values and standard errors of the means are shown (n = 3). Data were analyzed by two-way analysis of variance with a Bonferroni posttest (*, P < 0.05; ***, P < 0.001).