FIG 5.

Cell cycle distribution is not affected by knockdown of Rad51 or BRCA1. CIN612 cells were transduced with control shRNA (shScram) or Rad51 (shRad51) or BRCA1 (shBRCA1) shRNA containing lentiviral particles and either harvested at T₀ (undifferentiated) or differentiated in methylcellulose for 24 and 48 h. Cells from each time point were ethanol fixed, stained with propidium iodide (20 μg/ml), and analyzed for total cellular DNA content by flow cytometry. Graphed values are the averages of three independent experiments. Error bars represent means ± standard errors. Statistics were performed using a two-tailed t test comparing shScram to shRad51 and shBRCA1 for the G₂/M phase at each time point, with a P value of >0.05. N.S., not significant; MC, methylcellulose.