FIG 7.

Ifi27l2a restricts WNV replication in Mϕ cells but not in DCs or MEFs. (A) Bone marrow-derived Mϕ were infected with WNV (MOI, 0.01), and viral replication kinetics were followed for 72 h. A subset of Mϕ cells was pretreated with IFN-β (1 U/ml for 6 h) prior to infection. (B) Bone marrow-derived DCs were infected with WNV (MOI, 0.001), and viral kinetics were followed for 72 h. A subset of DCs was pretreated with IFN-β (10 U/ml for 6 h) prior to infection. (C) MEFs were infected with WNV (MOI, 0.01), and viral kinetics were followed for 72 h. A subset of MEFs was pretreated with IFN-β (10 U/ml for 6 h). The data were analyzed by a Student's t test for each time point for comparisons between treatment groups and are expressed as the log₁₀ median titers ± SEM as reflects pooled data from 3 to 4 independent experiments performed with three technical replicates per independent experiment for each cell type. Ranges of y-axis data are cell type dependent.