FIG 9.

Neuronal death within the cerebellum and brain stem of WNV-infected mice. Nine days following subcutaneous infection with WNV-NY, brains of selected mice with similar levels of virus were sectioned and stained for neurons (NeuN; green), cell death (as determined by TUNEL staining; red), and nuclei (DAPI; blue). Cerebellum (A) and brain stem (B) tissues were analyzed by confocal microscopy, and fields of view containing TUNEL staining were quantitated for numbers of events per field of view (C) (n = 4 mice per genotype, 2 sections per mouse, 2 fields of view per section). The relative numbers of TUNEL⁺ cells in WT and Ifi27l2a^−/− mice were analyzed by Student's t test, with bars indicating the means (*, P < 0.05; **, P < 0.005). Scale bars = 100 μm. Yellow arrowheads indicate examples of TUNEL⁺ nuclei. Inset boxes are higher-magnification images of areas denoted with yellow arrowheads.