Fig. 2.

Pairwise comparison of most significant cell shape parameters. Each panel shows the comparison between high metastatic (grey) and low metastatic (black) cell lines for a single significant parameter on all surfaces. The paired lines are indicated by letters as follows. D: DUNN and DLM8; K: K12 and K7M2; S: Saos2 and SAOS-LM7; M: MG63 and MG63.2. (A) Cell area, (B) cell major axis, (C) cell minor axis, (D) cell aspect ratio and (E) coefficient of variation (CV) of the radius from the center of mass to the hull. n=100 for each cell line on each surface. *P<0.05 by two-tailed t-test satisfying the Holm–Bonferroni criteria for all variables (Table S3).