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. 2016 Feb 12;5(3):289–299. doi: 10.1242/bio.013409

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Pairwise comparison of the most significant parameters of nucleus shape. Each panel shows the comparison between high metastatic (grey) and low metastatic (black) cell lines for a single significant parameter. The paired lines are indicated by letters as follows. D: DUNN and DLM8; K: K12 and K7M2; S: Saos2 and SAOS-LM7; M: MG63 and MG63.2. (A) Nuclear area, (B) major axis of the nucleus, (C) minor axis of the nucleus and (D) aspect ratio of the nucleus. n=100 for each cell line on each surface. *P<0.05 by two-tailed t-test satisfying the Holm–Bonferroni criteria for all variables (Table S3).