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. 2016 Feb 18;5(3):300–310. doi: 10.1242/bio.016402

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Rapid cellular changes in IMR90 cells after transduction with Ad-SOcMK. Alterations of morphology (Ab,d,f,h) and ALP expression (Bj,l,n,p) of Ad-SOcMK-transduced IMR90 cells with time after transduction are shown. Within one day, Ad-SOcMK-transduced cells show a different morphology (Ad) than Ad-GFP-transduced cells (Ac) with clear clustering (Af) and ALP expression by day 2 (Bn). In Ad-GFP-transduced cells, alterations of cell morphology (Aa,c,e,g) or ALP expression (Bi,k,m,o) are not seen. Ad-GFP or Ad-SOcMK adenoviruses were removed after one day (designated day 1), replaced with human ESC medium, and cell morphology was monitored. All phase contrast photomicrographs (A) and ALP cytochemistry images (B) were taken at 4× magnification. Representative micrographs of three independent experiments are shown.