Fig. 5.

Effects of overexpression of H1.1 or H1.5 on cell cycle progression. (A) Separation of H1 subtypes by HPLC. Total histones were isolated from density arrested cell cultures from the indicated lines and separated by HPLC as previously described (Brown et al., 1996). The subtypes present in each peak are indicated based on prior studies (Yellajoshyula and Brown, 2006). Estimates of the relative amounts of each subtype were determined by quantitation of the absorbance at 210 nm via integration of the area in each peak (see Table 3). (B) Cell cycle distribution after release from density arrest as determined by FACS. Results are the average of three independent experiments. (C) Individual FACS profiles from a representative experiment.