. Author manuscript; available in PMC: 2016 Mar 29.

Published in final edited form as: Exp Biol Med (Maywood). 2009 Feb 20;234(5):513–521. doi: 10.3181/0812-RM-369

Table 2. Selenium requirement hierarchy in testis of growing rats.

Biomarker	Minimum Requirement^a	Extent of Regulation^b

	(μg Se/g)
Testis weight	<0.005	Low (P = 0.40)
Gpx4 activity	<0.005	Low (P = 0.45)
Selenium	<0.016	Moderate (P = 0.066)^c
Sepw1 mRNA	<0.016	Moderate (P < 0.004)
Sepp1 mRNA	0.04	Moderate (P < 0.002)
Gpx1 activity	0.04	High (P < 0.0001)
Gpx1 mRNA	0.08	Moderate (P < 0.0001)

Minimum dietary Se requirement for the growing rat as determined for each indicated biomarker. Requirements are the minimum dietary Se necessary for the indicated parameter to reach plateau levels when Se-adequate weanling rats are fed these diets from weaning, as determined by breakpoint analysis as described in the text.

Susceptibility to Se regulation of the indicated biomarker in Se-deficient rat tissue in Se-deficient versus Se-adequate rats: high = 11-40.9% of Se-adequate; moderate = 41-70% of Se-adequate; Low = >70% of Se-adequate. These ranges of regulation are the same as applied in previous studies (7,8). Significance of regulation (P-value for ANOVA) indicated in parentheses.

Testis Se response curve was significantly hyperbolic (P < 0.0025).