Figure 1.

Effect of water removal on antigenicity. Spleen sections were antigen-retrieved (AR) and then immunostained for Ki-67, CD3 and bcl-2 in control conditions (control), after 1 hr at 60°C in a waterbath (60°C), after 1 hr at 60°C in a dry oven (oven), same as previous but soaked in 10% sucrose in water before oven-drying (oven + sucrose), after freeze-drying for 48 hr (F-D), or after drying in a desiccator (dry). The inset shows higher magnification detail of the lymphoid cells. Original magnification 10×; Scale, 100 µm. No nuclear counterstain.