Figure 4.

Rescue of antigenicity after drying with or without lactose. Top: Tonsil sections, antigen-retrieved (AR), were immunostained for Ki-67, CD79a and bcl-2 in control conditions (control), after 1 hr at 60°C in a dry oven (oven dry), and after a second AR process performed after being oven-dried, before immunostaining (oven dry + AR). Bottom: Tonsil sections were immunostained for Ki-67 and bcl-2 with a routine protocol: freshly after being cut (control); after dewaxing, AR, soaking in lactose, oven-drying, and storage at room temperature for 2 months (2 mo + lactose 10%); or treated as before, but subjected to AR again before immunostaining (2 mo + lactose 10% + AR). Original magnification 10×; Scale, 100 µm. Light nuclear counterstain.